Effects of 5-fluorouracil with 1-methyl tryptophan on myeloid-derived suppressor cells in gastric cancer bearing 615 mice

VernacularTitle:1-甲基色氨酸与5-氟尿嘧啶联合干预对荷胃癌小鼠髓源性抑制细胞的影响
Author: Gai WANG ; Huiqin SHEN ; Xirun WU ; Xiaoli LIU ; Guiyun KANG ; Zheping LI ; Qi WANG
Publication Type:Journal Article
Keywords: Stomach neoplasms; Tryptophan; Fluorouracil; Myeloid-derived suppressor cells
From: Chinese Journal of General Surgery 2014;29(6):464-466
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of 1-MT in combination with 5-FU on the number of myeloid-derived supressor cells (MDSC) in gastric carcinoma beating mice.Methods By using the lipofectamine TM 2000,the eukaryotic expression plasmid pcDNA3.1-IDO and empty vector pcDNA3.1 (+) were transfected in a MFC cell line.Animal model of gastric cancer bearing 615 mice were established to give respectively normal saline (NS),1-MT,5-FU and 1-MT + 5-FU therapy.Flow cytometry and immunofluorescence were used to analyze MDSC expression in tumor tissue.Result (1) The expression of MDSC was detected in transplanted tumor.(2)The expression of MDSC in gastric cancer beating 615 mice (49.8％ ± 1.1％) was higher than the normal group (1.2％ ± 0.3％) (P ＜ 0.05).(3) Compared with normal group the MDSC expression in 1-MT + 5-FU group (18.5 ％ ± 0.5 ％) decreased significantly (P ＜ 0.05).Conclusions Combining 1-MT with 5-FU can reduce the number of MDSC in gastric cancer bearing 615 mice,improving tumor microenvironment.