Expression of MAP4K4 in bladder cancer and its biological effect on human bladder cancer T24 cells
10.3760/cma.j.issn.1000-6702.2014.06.019
- VernacularTitle:MAP4K4在膀胱癌中的表达及对膀胱癌细胞株T24生物学行为的影响
- Author:
Baojie MA
;
Haibo LI
;
Changwen ZHANG
;
Qiliang CAI
;
Gang LI
;
Yong XU
- Publication Type:Journal Article
- Keywords:
Mitogen-activated protein kinase kinase kinase kinase 4;
Urinary bladder neoplasms;
shRNA;
Gene silencing;
Oligonucleotide array sequence analysis
- From:
Chinese Journal of Urology
2014;35(6):469-472
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of MAP4K4 targeted shRNA on biological characteristics such as proliferation,invasiveness,and apoptosis in human bladder cancer cell.Methods Differentially expressed genes was screened out through cDNA microarray analysis in 5 pairs of fresh-frozen muscle-invasive bladder cancer(MIBC) and adjacent normal tissue obtained from radical cystectomy.Combining the results of genechip and literature review,MAP4K4 was picked up for further analysis.To verify the result of microarray analysis,16 pairs of fresh muscle-invasive bladder cancer (MIBC) and adjacent tissues were assessed for the expression of MAP4K4 mRNA and protein through RT-PCR,qRT-PCR and Western-blot.T24 cell line was stably trasfected with MAP4K4 targeted shRNA and control shRNA,respectively.The effects of MAP4K4 silencing on proliferation,invasiveness and apoptosis of T24 cells transfected with MAP4K4 targeted shRNA and control shRNA were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT),transwell and flowcytometry (FCM) assay.Results MAP4K4 was overexpressed in muscle invasive bladder cancer than in normal tissue.Down regulation of MAP4K4 expression decreased bladder cancer cell proliferation(MAP4K4-targeted versus control,P＜0.001),invasiveness(MAP4K4-targeted versus control,P=0.004)and promoted cell apoptosis(MAP4K4-targeted versus control,P=0.023).Conclusions MAP4K4 is overexpressed in muscle invasive bladder cancer than in normal tissue.Down-regulation of MAP4K4 expression inhibits the invasive ability of bladder cancer.Therefore,MAP4K4 might be a potential therapeutic target for bladder cancer.
