Effects of high glucose on the proliferation and differentiation of primary osteoblasts
10.3760/cma.j.issn.0254-9026.2014.06.029
- VernacularTitle:高糖对原代成骨细胞增殖分化的影响
- Author:
Yonghui DONG
;
Fei XU
;
Fengjin GUO
;
Anmin CHEN
;
Yu DU
;
Shilong HUANG
- Publication Type:Journal Article
- Keywords:
Glucose;
Osteoblasts;
Cell proliferation;
Cell differentiation
- From:
Chinese Journal of Geriatrics
2014;33(6):665-667
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of different concentrations of glucose on the proliferation and differentiation of primary osteoblasts.Methods The identification of mouse primary osteoblasts was performed by alkaline phosphatase (ALP)staining and Von Kossa staining.Treating osteoblasts with different dose of glucose (5.5,15.5,25.5 mmol/L),the osteoblasts proliferation,ALP staining,and Runx2,OB markers ALP and OCN mRNA expression were observed.Real-time PCR was used for the determination of Runx2,OB markers ALP and OCN mRNA expression.Results With the increasing glucose concentrations,the osteoblasts cell proliferation was decreased.Compared with 5.5 mmol/L normal glucose,the ALP staining in 15.5 mmol/L group and 25.5 mmol/L group were decreased.The expressions were decreased by (36.7±6.2)% and (38.3±2.2)% in Runx2 mRNA,(26.7±7.2)% and (40.4±4.3)% in OCN mRNA respectively.ALP in 15.5 mmol/L group was reduced by (33.3±10.2)%,but increased by(50.8±10.4) % in 15.5 mmol/L group.Conclusions High glucose may decrease osteoblasts proliferation and activity,which may be one of the key pathogenesis factors of diabetic osteoporosis.