Comparative evaluation of disk diffusion and E-test methods for clarithromycin susceptibility testing of Helicobacter pylori
10.3760/cma.j.issn.1674-2397.2014.03.006
- VernacularTitle:幽门螺杆菌克拉霉素纸片扩散法药敏试验标准化研究
- Author:
Xianchu LIN
;
Lan LI
;
Hongzhang LI
;
Ningmin YANG
;
Tiangan YANG
;
Wanli ZENG
;
Qiang CHEN
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
Clarithromycin;
Disk diffusion antimicrobial tests;
E-test
- From:
Chinese Journal of Clinical Infectious Diseases
2014;7(3):218-221
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare disk diffusion with E-test methods for clarithromycin susceptibility testing of Helicobacter pylori (H.pylori).Methods A total of 44 strains of H.pylori were isolated from gastric mucosa biopsy from patients undergoing gastroscopic examination.Disk diffusion and E-test methods were used for clarithromycin susceptibility testing of H.pylori.The agreement of disk diffusion and E-test was assessed by linear regression analysis.Results The minimum inhibitory concentration (MIC) tested by E-test method ranged from 0.016 to 256 μg/mL,and drug resistance was observed in 12(27.3％) isolates.In range of 0-35 mm of inhibition diameter,the results of disk diffusion method were correlated well with the MICs obtained by E-test method (r2 =0.91,P ＜0.01).Regression analysis showed that with inhibition diameters≥ 18 mm as considered sensitive to clarithromycin and ≤ 15 mm as resistant,the agreement was 100％ between two methods.Conclusion The disk diffusion method is equivalent to the E-test method for clarithromycin susceptibility testing of H.pylori,which can be an alternative method for clinical application.
