Anti-tumor effect of ethanol extracts from Thymus quinquecostatus Celak on human leukemia cell line
- Author:
Zhenxiao SUN
;
Yinghui ZHANG
;
Shuang CHENG
;
Qingwen MA
;
Shanli GUO
;
Jinbao ZHANG
- Publication Type:Journal Article
- From:
Journal of Integrative Medicine
2005;3(5):382-5
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To screen the anti-tumor fraction of ethanol extracts from Thymus quinquecostatus Celak and investigate its anti-tumor effect on human leukemia cell line. METHODS: Ethyl acetate, n-butanol and acetone fractions were separated from the ethanol extracts of wild Thymus quinquecostatus Celak. Growth inhibiting effects of these extracts on human leukemia cell lines K562 and HL-60 were determined by live cell counting and cell growth curve analysis. The possible anti-tumor mechanism was studied by morphological analysis with norcantharidin as a positive control. RESULTS: Ethyl acetate fraction could significantly inhibit the proliferations of K562 and HL-60 cells, and the inhibiting effect depended on the concentration of ethyl acetate fraction. Ethyl acetate fraction could induce apoptosis of K562 and HL-60 cells. The n-butanol and acetone fractions had no significant inhibiting effect on K562 and HL-60 cells. CONCLUSION: Ethyl acetate fraction is the major anti-tumor fraction in ethanol extracts from Thymus quinquecostatus Celak.
