Accelerated and Exacerbated Effects of High Dietary Fat on Neuronal Damage Induced by Transient Cerebral Ischemia in the Gerbil Septum.
10.3803/EnM.2014.29.3.328
- Author:
Seung Hwan CHEON
1
;
Bing Chun YAN
;
Bai Hui CHEN
;
Joon Ha PARK
;
Ji Hyeon AHN
;
In Hye KIM
;
Jae Chul LEE
;
Yoo Seok PARK
;
Min Joung KIM
;
Yun Lyul LEE
;
Jun Hwi CHO
;
Moo Ho WON
Author Information
1. Department of Emergency Medicine, Kangwon National University School of Medicine, Chuncheon, Korea. cjhmd@kangwon.ac.kr
- Publication Type:Original Article
- Keywords:
Diet, high-fat;
Ischemic attack, transient;
Glial activation;
Septohippocampal nucleus
- MeSH:
Astrocytes;
Blood Glucose;
Body Weight;
Brain Ischemia;
Cholesterol;
Diet;
Diet, High-Fat;
Dietary Fats*;
Gerbillinae*;
Gliosis;
Immunohistochemistry;
Ischemia;
Ischemic Attack, Transient*;
Microglia;
Models, Animal;
Neurons*;
Obesity;
Triglycerides
- From:Endocrinology and Metabolism
2014;29(3):328-335
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Obesity induced by high-fat diet (HFD) is one of the most widespread metabolic disorders in current society. However, there has been little research regarding the effects of HFD-induced obesity in the septa of animal models of cerebral ischemia. Therefore, in the present study, we investigated septal effects of HFD on neuronal damage and gliosis induced by transient cerebral ischemia. METHODS: Body weight, blood glucose levels and serum lipid profiles levels were measured both in the normal diet (ND) and HFD-group. We also investigated the effects of ND and HFD on neuronal damage and gliosis in the septum after transient cerebral ischemia using immunohistochemistry. RESULTS: The levels of blood glucose, serum triglyceride, and total cholesterol were significantly increased in the HFD-fed gerbils compared with the ND-fed gerbils, although body weight was not significantly changed after HFD feeding. In the ND-fed gerbils, ischemia-induced neuronal damage was found in the septohippocampal nucleus (SHN) of the septum 7 days after ischemia. In the HFD-fed gerbils, ischemia-induced neuronal damage in the SHN was much more severe compared with that of the ND-fed gerbils 4 and 7 days after ischemia. In addition, we found that ischemia-induced glial activation including astrocytes and microglia was accelerated and exacerbated in the HFD-fed gerbils compared with that in the ND-fed gerbils. CONCLUSION: These results indicate that HFD can lead to much more severe effects in ischemia-induced neuronal damage/death in the septum after ischemia-reperfusion, and that it may be associated with accelerated change in glial activation.
