Polarized and Stage-Dependent Distribution of Immunoreactivity for Novel PDZ-Binding Protein Preso1 in Adult Neurogenic Regions.
10.3803/EnM.2014.29.3.349
- Author:
Eun Soo LEE
1
;
Woon Ryoung KIM
;
Younghwa KIM
;
Hyun Woo LEE
;
Hyun KIM
;
Woong SUN
Author Information
1. Department of Anatomy, BK21 Program, Korea University College of Medicine, Seoul, Korea. woongsun@korea.ac.kr
- Publication Type:Original Article
- Keywords:
Preso1;
Frmpd4;
Cell polarity;
Subventricular zone;
Dentate gyrus;
Adult neural stem cells
- MeSH:
Adult*;
Animals;
Asymmetric Cell Division;
Axons;
Brain;
Cell Differentiation;
Cell Lineage;
Cell Polarity;
Cytosol;
Dendritic Spines;
Dentate Gyrus;
Humans;
Immunohistochemistry;
Mice;
Neural Stem Cells;
Neurons;
Rivers
- From:Endocrinology and Metabolism
2014;29(3):349-355
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Adult neural stem cells have the potential for self-renewal and differentiation into multiple cell lineages via symmetric or asymmetric cell division. Preso1 is a recently identified protein involved in the formation of dendritic spines and the promotion of axonal growth in developing neurons. Preso1 can also bind to cell polarity proteins, suggesting a potential role for Preso1 in asymmetric cell division. METHODS: To investigate the distribution of Preso1, we performed immunohistochemistry with adult mouse brain slice. Also, polarized distribution of Preso1 was assessed by immunocytochemistry in cultured neural stem cells. RESULTS: Immunoreactivity for Preso1 (Preso1-IR) was strong in the rostral migratory stream and subventricular zone, where proliferating transit-amplifying cells and neuroblasts are prevalent. In cultured neural stem cells, Preso1-IR was unequally distributed in the cell cytosol. We also observed the distribution of Preso1 in the subgranular zone of the hippocampal dentate gyrus, another neurogenic region in the adult brain. Interestingly, Preso1-IR was transiently observed in the nuclei of doublecortin-expressing neuroblasts immediately after asymmetric cell division. CONCLUSION: Our study demonstrated that Preso1 is asymmetrically distributed in the cytosol and nuclei of neural stem/progenitor cells in the adult brain, and may play a significant role in cell differentiation via association with cell polarity machinery.
