Identification of bufadienolides profiling in cinobufacino by HPLC-DAD-FT-ICR-MS method.
- Author:
Junqiu LIU
;
Nan SI
;
Jian YANG
;
Haiyu ZHAO
;
Baolin BIAN
;
Hongjie WANG
- Publication Type:Journal Article
- From:
Acta Pharmaceutica Sinica
2014;49(2):244-8
- CountryChina
- Language:Chinese
-
Abstract:
Cinobufacino injection is a significant anti-tumor medicine for the treatment of various tumors in clinic, which was made from water extraction of the skin of Bufo bufo gargarizans. In present paper, HPLC-DAD-FT-ICR-MS method was used to identify the major bufadienolides in cinobufacino for the first time. Solid-phase extraction with dichloromethane and silica was used to enrich the total bufadienolides in cinobufacino. Based on the UV and high resolution MS/MS data, 33 bufadienolides were analyzed and characterized. Among them, eight compounds were identified by comparing with standard references unambiguously. This study elucidated the major bufadienolides in cinobufacino, which provided material foundation of cinobufacino and will be benefit for the further pharmacological research.