Study on UHPLC Fingerprints of Psoralea corylifolia L.
10.11842/wst.2014.04.033
- VernacularTitle:基于UHPLC技术的补骨脂指纹图谱研究
- Author:
Xiaochuan CHEN
;
Ziwei LI
;
Huixian TANG
;
Xiaomei ZHANG
;
Na LI
- Publication Type:Journal Article
- Keywords:
Psoralea corylifolia L.;
fingerprints;
relative retention time (RRT);
ultra high performance liquid chromatography ( UHPLC )
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(4):865-868
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to establish the ultra high performance liquid chromatography (UHPLC) fingerprints of Psoralea corylifolia L. The separation was achieved on a Shim-pack XR-ODS Ⅲ column (50 mm í 2.0 mm, 1.6μm) by gradient elution with acetonitrile-0.2% glacial acetic acid solution as the mobile phase. The flow rate was 0.1 mL·min-1 and the measurement wavelength was 246 nm. The temperature of the column was 45oC. The results showed that the UHPLC fingerprint of P. corylifolia L. was established and 10 characteristic common peaks were found, among which 6 peaks were recognized by comparing with reference substances. It was concluded that the method was rapid, reliable and reproducible. The established fingerprint can provide references for the study of sub-stance basis and quality control of P. c orylifolia L.
