An analysis on results of different detection methods for hepatitis B virus surface antigen

VernacularTitle:不同方法检测乙型肝炎病毒表面抗原的结果分析
Author: Jiang HE
Publication Type:Journal Article
Keywords: hepatitis B surface antigens; enzyme-linked immunosorbent assay; gold labeled immunochromatographic assay; time resolved fluoroimmunoassay
From: International Journal of Laboratory Medicine 2014;(9):1164-1165
CountryChina
Language:Chinese
Abstract: Objective To analyze the results of gold labeled immunochromatographic assay (GICA ) ,enzyme-linked immunosor-bent assay (ELISA ) and time resolved fluoroimmunoassay (TRFIA ) detection for hepatitis B virus surface antigen (HBsAg ) . Methods GICA ,ELISA and TRFIA were employed to conduct the HBsAg testing in 11 058 specimens .Results The HBsAg posi-tive rates of GICA ,ELISA and TRFIA detection in 11 ,058 specimens were 15 .52% ,15 .64% and 15 .95% ,respectively ,with no statistically significant difference when pairwise comparison performed (P>0 .05) .In specimens which results of TRFIA quantita-tive detection was 0 .2- <2 .0 ng/mL ,the diagnostic compliance rates of GICA ,ELISA were 25 .0% and 75 .0% ,respectively .Con-clusion The diagnostic compliance rates of GICA ,ELISA detection in specimens with low concentration HBsAg are deceased .