Experimentla study on the expression of albumin in blood-retinal barrier injury of oxygen-induced retinopathy
10.3760/cma.j.issn.1008-1372.2014.04.002
- VernacularTitle:视网膜白蛋白检测在评价小鼠血-视网膜屏障中的实验研究
- Author:
Xiaofeng TIAN
;
Xiaobo XIA
- Publication Type:Journal Article
- Keywords:
Retinal neovascularization;
Albumins/metabolism;
Blood-retinal barrier
- From:
Journal of Chinese Physician
2014;(4):435-438
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate expressions of albumin during retinal neovascularization in a murine model of oxygen -in-duced retinopathy.Methods C57BL/6J mice were exposed to (75 ±2)%oxygen for 5 days and returned to room air to induce retinal neovascularization .Retinal neovascularization was observed by fluorescence angiography , and was quantified by counting the endotheli-al nuclei protruding into the vitreous cavity after hematoxylin-eosin staining.The blood-retina barrier was evaluated by Western blot a-nalysis of extravasated albumin in the retina .Results In fluorescence angiograms , irregular neovascularization and fluorescein leakage was observed surrounding the unperfused areas in the hypoxic group .Compared to the normoxic group , there was a significant increase in both-side retinal albumin protein levels in the hypoxic group at P 13, P15 , and P17.Conclusions Albumin expression was in-creased in murine retina under hypoxia , and might be used to detect blood-retinal barrier damage of oxygen-induced retinopathy .
