Analysis on chlorogenic acid of chaenomeles speciosa and its correlations with anticancer by high performance liquid chromatography-mass spectrometry
10.3969/J.ISSN.1672-8270.2014.05.002
- VernacularTitle:液相色谱-质谱联用法分析木瓜中绿原酸与抗癌活性成分的相关性研究
- Author:
Guang ZHAO
;
Jun LI
;
Xuejing DU
- Publication Type:Journal Article
- Keywords:
Chaenomeles speciosa;
HPLC-MS;
Chlorogenic acid
- From:
China Medical Equipment
2014;(5):4-6
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze chlorogenic acid of Chaenomeles speciosa by high performance liquid chromatography-mass spectrometry. Methods:A method for determining chlorogenic acid of Chaenomeles speciosa by HPLC-MS is described. The operating conditions were Diamonsil C18 column (5μm, 4.6×250 mm) at room temperature, acetonitrile-0.05%phosphoric acid solution as mobile phase by step gradients elution at a flow of 0.8 ml/min and UV detection at 325 nm. Results: The content of chlorogenic acid of Chaenomeles speciosa is 1.3 mg/g (RSD 0.4%), respectively. Conclusion:Chlorogenic acid was detected from Chaenomeles speciosa, which may be related to its inhibition of FAS and the potential role of anticancer.
