Study on gallic acid induced human hepatoma SMMC-7721 cells apoptosis and its mechanism
10.3969/j.issn.1001-1978.2014.05.015
- VernacularTitle:没食子酸诱导人肝癌细胞SMMC-7721凋亡机制的探讨
- Author:
Muhan LI
;
Mingyan WANG
;
Fengming ZHAO
;
Haibin CHEN
;
Hongguang ZHOU
;
Qingchun ZHAO
;
Wenting LI
;
Mianhua WU
- Publication Type:Journal Article
- Keywords:
gallic acid;
hepatoma;
SMMC-7721 cells;
proliferation;
cell apoptosis;
tumor suppressor gene p53
- From:
Chinese Pharmacological Bulletin
2014;(5):657-661
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the proliferative effect and the apoptosis of human hepatoma SMMC-7721 cells induced by gallic acid ( GA ) , and its underlying mechanism. Methods SMMC-7721 cells were cul-tured in vitro. MTT assay was used to observe the pro-liferation of SMMC-7721 cells induced on GA 24 , 48 , 72 h. The morphological and ultra structural changes of the SMMC-7721 cells were observed by inverted micro-scope and transmission electron microscope respective-ly. Annexin V-FITC/PI staining was used to quantify the percentages of apoptosis in the total cell popula-tion. The expression of p53 mRNA was investigated by RT-PCR. Western blot was used to determine the pro-tein expression of p53. Results GA(6. 25~50 μmol ·L-1 ) markedly inhibited the activity of proliferation and induced apoptosis of SMMC-7721 cells after 48 h in a dose-dependent manner. GA significantly induced cell nuclear condensation and fragmentation. RT-PCR and Western blot results showed that GA could improve the expression of p53 mRNA and protein. Conclusion GA can inhibit the proliferation of human hepatoma SMMC-7721 cells and induce cells apoptosis. The mechanism may be associated with improving tumor suppressor gene p53 expression.
