Atorvastatin attenuates activation and injury of human microvascular endothelial cells induced by oxidized low density lipoprotein
10.3969/j.issn.1001-1978.2014.05.020
- VernacularTitle:阿托伐他汀减轻氧化型低密度脂蛋白导致的人微血管内皮细胞活化和损伤
- Author:
Min LI
;
Ling SUN
;
Hongling LI
;
Qianyun SUN
- Publication Type:Journal Article
- Keywords:
endothelial cell;
oxidized low density lip-oprotein;
nuclear factor κB;
atorvastatin;
inflamma-tion;
adhesion molecule
- From:
Chinese Pharmacological Bulletin
2014;(5):679-683
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of atorvas-tatin ( ATV) on activation and injury of microvascular endothelial cells induced by oxidized low density lipo-protein ( ox-LDL) . Methods Cultured human micro-vascular endothelial cells were pre-incubated with ATV for 24 h prior to exposure of endothelial cells to ox-LDL. After exposure of endothelial cells to ox-LDL, the cell viability was measured by MTT method, LDH in supernatants was determined by enzyme activity as-say kit, ICAM-1 in supernatants was assayed by using ELISA method, phosphorylation of NF-κB p65 was de-tected by western blot analysis, transcriptional activity of NF-κB signal pathway was measured by employing dual-luciferase reporter assay system. Results Hu-man microvascular endothelial cells were activated and injured by ox-LDL. Inhibition of the cell viability, re-lease of LDH, expression of ICAM-1, phosphorylation of NF-κB p65 , and up-regulated transcriptional activity of NF-κB induced by ox-LDL were attenuated by ATV. Conclusion ATV can significantly inhibit the activa-tion and injury of human microvascular endothelial cells induced by ox-LDL, and that may be related to inhibition of phosphorylation and transcriptional activity of NF-κB.
