Inhibitory effect of mastoparan-like peptide from wasp (Vespa magnifica) venom on angiogenesis
10.3969/j.issn.1001-1978.2014.05.028
- VernacularTitle:大胡蜂肥大细胞脱粒肽对血管生成的抑制作用
- Author:
Wenjun LIU
;
Xueqing XU
- Publication Type:Journal Article
- Keywords:
mastoparan;
chicken chorioallantoic membrane;
Vespa magnifica;
angiogenesis;
human umbilical vein endothelial cells;
antimicropeptide;
tumor
- From:
Chinese Pharmacological Bulletin
2014;(5):715-718
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the anti-angiogenic effect of 12a, the mastoparan-like peptide from wasp ( Vespa magnifica ) venom. Methods The tube for-mation and proliferation of human umbilical vein endo-thelial cells ( HUVEC ) and chicken chorioallantoic membrane ( CAM) model were used to observe the an-ti-angiogenic effect of 12a in vitro and in vivo, respec-tively. Results In the CAM model, 0. 1 μg or 1 μg of 12 a could markedly inhibit angiogenesis induced by 0. 2 μg rh-bFGF with heavy loss of color, decreasing density and obscure frame of the vessels. The inhibi-tion rates of angiogenesis were 60 . 2 % for 0 . 1 μg and 90. 3 % for 1 μg, respectively. Accordingly, in HU-VEC culture experiment, the proliferation and angio-genesis of HUVEC treated by 1 mg·L-1 and 10 mg· L-1 of 12 a were decreased by 55. 4 %, 39. 3 % and 51. 6 %, 26. 7 %, respectively. Conclusion 12a has a significant anti-angiogenic effect in a concentra-tion-dependent manner.
