Study on Quality Standard for Compound Heishen Pills
- VernacularTitle:复方黑参丸的质量标准研究
- Author:
Jin ZHANG
;
Hongda MA
;
Jun GAO
;
Bei HU
;
Guobing SHI
- Publication Type:Journal Article
- Keywords:
Compound Heishen pills;
Quality standard;
Harpagoside;
Cinnamic acid;
HPLC
- From:
China Pharmacist
2014;(5):716-718
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the quality standard for compound Heishen pills. Methods: Scrophulariae Radix, Radix et Rhizoma and Belamcancae Rhizoma were identified by TLC. HPLC was used to determine the content of harpagoside and cinnamic acid in Scrophulariae Radix on a Welchrom-C18 column using methanol-acetonitrile-1% ethylic acid (8∶21∶71) as the mobile phase. The flow rate was 1. 0 ml·min-1 . The column temperature was at 30℃ and the detection wavelength was set at 278 nm. Results:The TLC method had good specificity without interference from negative control. The linear range of harpagoside was 1. 32-65. 80μg·ml-1 with the average recovery of 98. 06%(RSD=2. 16%),and that of cinnamic acid was 0. 38-19. 20 μg·ml-1 with the average recovery of 98. 78%(RSD=1. 34%). RSDs of precision, stability and reproductibility tests were all below 2%. Conclusion: The established method is accurate, feasible and reproducible. It can be used in the quality control of compound Heishen pills.
