The expression of glutamate receptor in hippocampus of neonatal rats with hypoxic-ischemic brain damage
10.3969/j.issn.1000-3606.2014.04.020
- VernacularTitle:谷氨酸受体在新生大鼠缺氧缺血脑损伤海马组织中的表达
- Author:
Xiaojuan TANG
;
Bin SUN
;
Ying WANG
;
Xin DING
;
Lun YU
;
Lixiao XU
;
Xing FENG
- Publication Type:Journal Article
- Keywords:
hypoxic-ischemic brain damage;
glutamate receptors;
autophagy;
hippocampus;
rat
- From:
Journal of Clinical Pediatrics
2014;(4):368-371
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of Ca-A/K channel-related molecules glutamate receptor 2 and 1(GluR2/1) in hippocampus tissues of neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods A total of 60 7-day-old Sprague-Dawley rats were randomly divided into sham operation group and HIBD group. Hippocampal tissues were obtained at 0 h, 1 h, 6 h, 24 h, 48 h and 72 h after HIBD. The expression of GluR2, GluR1 and autophagy marker protein Beclin-1, LC3 were detected by Western blot assay. Results Edema and focal softening and necrosis were observed 6 h after HIBD in the brains of neonatal rats. Compared with Con group, at each time point, the expression levels of GluR2 were lower while the levels of GluR1, Beclin-1 and LC3 were higher significantly in HIBD group (P<0.05). The protein levels of LC3, Beclin-1, GluR1 and GluR2 in hippocampus tissues of HIBD group were significantly different among different time points after the estab-lishment of HIBD model (F=10.65~701.14, P<0.01). The protein level of GluR2 was decreased from 1 h to 24 h after HIBD and reached the lowest level at 24 h. The levels of GluR1, Beclin-1 and LC3 were increased at 6 h, plateaued at 24 h and remained there until 48 h. The levels of these proteins returned back to the initial level at 72 h. Conclusions Ca-A/K channel-related mol-ecules GluR2 and GluR1 play important roles in the autophagic cell death of hippocampus tissues in neonatal rats with hypoxic-ischemic brain damage.
