Expression of CD24 in cardiac adenocarcinoma and its clinical significance
10.13315/j.cnki.cjcep.2014.04.004
- VernacularTitle:贲门腺癌中CD24的表达及临床意义
- Author:
Yanning CHEN
;
Meng ZHANG
;
Xiaoling WANG
;
Huichai YANG
;
Yongjun WANG
;
Yueping LIU
- Publication Type:Journal Article
- Keywords:
cardiac adenocarcinoma;
CD24;
cancer stem cells;
clinicopathological features
- From:
Chinese Journal of Clinical and Experimental Pathology
2014;(4):371-375
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the expression of CD24 in cardiac adenocarcinoma, and to explore the relationship between the two molecules and other clinicopathological features. Methods The expression of CD24 in 140 cases of cardiac adenocarcinoma was detec-ted by using immunohistochemistry. And the relationship between CD24 and clinicopathological features, adenocarcinoma biological be-havior were analyzed, total RNAs were extracted from 26 cases of fresh carcinoma tissues and adjacent mucosa, each groups were divid-ed by different clinical staging, different degree of differentiation. The expression of CD24 was detected by using RT-PCR and the re-sults were shown as relative quantity. The difference of CD24 expression between two groups was further analyzed and the invasion of both groups was compared. Results Immunohistochemically, the expression of CD24 was significantly higher in carcinoma tissues than that in adjacent mucosa (P<0. 05), and those expression was associated with tumor differentiation (P<0. 05). CD24 was also closely related with the invasion, lymph node metastasis, clinical stages and nerves involvement (P<0. 05), while no relation with age, gender, vascular blots and residual situation (P>0. 05), the expression of CD24 in two pathological types showed that Lauren diffuse type was higher than intestinal type, the expression of CD24 was different among tubular adenocarcinoma, polypoid adenocarci-noma, mucinous adenocarcinoma and low adhesion adenocarcinoma (WHO classification) (P<0. 05). RT-PCR results demonstrated that the expression of CD24 was markedly higher in carcinoma tissues than those in adjacent mucosa ( P<0. 05 ) . The expression of CD24 in poorly differentiated adenocarcinoma was significantly higher than those in intermediate/highly differentiated adenocarcinoma (P<0. 05). CD24 expression was also associated with clinical stages. The relative quantity from stageⅠtoⅣwas progressively ris-ing (P<0. 05). Conclusions The expression of CD24 is closely related with the tumor differentiation, invasion, lymph node metas-tasis and clinical staging, suggesting that CD24 may be involved in the progression of cardiac adenocarcinoma, and the patients with higher CD24 expression may have poorer prognosis.
