Molecular mechanism of As2 O3 in suppressing metastasis of esophagus carcinoma cells

VernacularTitle:三氧化二砷抑制食管癌细胞侵袭能力的机制研究
Author: Canfeng LIN ; Yanjie YOU ; Shubo XIN ; Wenmei LI ; Haijun LI ; Xiaohui ZHANG ; Yonggang RAN
Publication Type:Journal Article
Keywords: Esophageal neoplasms/pathology; Antineoplastic agents/pharmacology; Arsenicals/pharmacology; Tumor cells,cultured/drug effects; Cell proliferation; Neoplasm invasiveness
From: Journal of Chinese Physician 2014;(4):465-467
CountryChina
Language:Chinese
Abstract: Objective To investigate the molecular mechanism of As 2 O3 in suppressing metastasis of esophagus carcinoma cells.Methods The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay, adhesion and invasion assay were performed to observe the inhibitory effect of As 2 O3 on proliferation and metastasis of esophagus carcinoma cells .The expressions of matrix metalloproteinases ( MMP)2, MMP9, E-cadherin, and protein tyrosine phosphatase receptor-type O ( PTPRO) were analyzed with Western blot .Results Exposure to As 2 O3 significantly presented suppressive functions on growth and metastasis of esophagus carcinoma cells in a dose-dependent manner ( P <0.01 ) .Additionally , MMP2 and MMP9 expressions were increased after treatment with casticin ( P <0.01 ) , whereas E-cadherin and PTPRO expressions were down-regulated ( P <0.01 ) .Conclusions As2 O3 had a significant function to inhibit proliferation and metastasis of esophagus carcinoma cells .