Research on the serum level of microRNA-224 in hepatocellular carcinoma patients and its clinical diagnostic significance
10.3969/j.issn.1000-8179.20140061
- VernacularTitle:肝细胞肝癌患者血清microRNA-224水平变化及其临床诊断意义的研究
- Author:
Hua ZHANG
;
Xuyi CHEN
;
Bo YANG
;
Minghua ZHANG
;
Renjie WANG
- Publication Type:Journal Article
- Keywords:
hepatocellular carcinoma;
miR-224;
real-time quantitative PCR;
diagnostic
- From:
Chinese Journal of Clinical Oncology
2014;(9):576-579
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the serum level of miR-224 in hepatocellular carcinoma (HCC) patients and its clinical diag-nostic significance. Methods:The serum level of miR-224 was detected by real-time quantitative PCR. This study included 42 cases of patients with HCC, 36 patients with liver cirrhosis (LC), 55 patients with chronic hepatitis B (CHB), and 40 healthy persons (NC). The relative expressions of miR-224 were calculated. The receiver operating characteristic (ROC) curves were analyzed to determine the sensitivity and specificity of miR-224 expression levels in HCC diagnosis. Results:Result shows that the relative miR-224 expression was higher in the serum of HCC patients than that in the CHB, LC, and NC groups. The difference was statistically significant (P<0.05 or P<0.01). The relative miR-224 expression in the serum of HCC patients and alpha fetoprotein (AFP) were positively correlated (P<0.05). By comparison, the tumor size, TNM stage, tumor differentiation, and lymph node metastasis were not correlated with mirR-224 expression (P>0.05). ROC analysis shows that the best critical value of the relative expression levels of miR-224 was 3.47, with sensi-tivity of 82.2%, specificity of 92.8%, and area under the curve of 0.935. Conclusion:The serum level of miR-224 in HCC patients has high specificity, and miR-224 has great potential to become a new serological marker for the diagnosis of HCC.
