Adiponectin reduces apoptosis by improving myocardial anti-oxidative activity after myocardial ischemia/reperfusion injury in diabetic rats
10.3969/j.issn.1001-1978.2014.05.008
- VernacularTitle:脂联素通过增强糖尿病大鼠心肌缺血/再灌注损伤后心肌抗氧化能力减少细胞凋亡
- Author:
Jia GUO
;
Yunfei BIAN
;
Chuanshi XIAO
;
Zhidong LI
- Publication Type:Journal Article
- Keywords:
adiponectin;
rat;
diabetes mellitus;
is-chemia /reperfusion injury;
cardiomyocyte;
antioxida-tive ability
- From:
Chinese Pharmacological Bulletin
2014;(5):623-627
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of adiponectin ( APN) on myocardial ischemia reperfusion( IR) injury in diabetic rats and to explore the role of oxidation-an-tioxidation system. Methods Male Sprague Dawley rats were randomly divided into control group( NS) , IR group ( NIR ) , diabetes group ( DS ) , DS + IR group (DIR), DS +APN +IR group(APN). Experimental diabetes was induced in the animals by a single intrap-eritoneal injection of streptozotocin at a dose of 55 mg ·kg-1 . The IR and NIR group were subjected to myo-cardial I/R injury. APN group was administered APN through intravenous injection 10 min before the reper-fusion, the others were administered normal saline. The rats were considered diabetic and used for the study only if their glucose levels were higher than 15 mmol·L-1. Results All the diabetic rats exhibited increased levels of blood glucose and reduction of body weight ( P <0. 05 ) . Compared with those of NS and DS groups, the myocardial infarct size, cardiomyocyte apoptosis, MDA concentration and ROS in NIR and DIR groups were remarkably increased, activities of SOD and NO were decreased(P<0. 05 or P<0. 01). APN decreased oxidative stress product generation and myocardial apoptosis induced by diabetic myocardial I/R injury ( P <0. 05 ) . Conclusion APN exerts pro-tective effect on myocardial I/R injury in diabetic rats through anti-oxidative mechanism.
