Effect of ginkgolide B on junctional proteins in oxidized LDL-stimulated human umbilical vein endothelial cells
10.3969/j.issn.1001-1978.2014.05.013
- VernacularTitle:银杏内酯B对内皮细胞连接蛋白的影响及其分子机制研究
- Author:
Xueqing LIU
;
Beidong CHEN
;
Li BAO
;
Wei WU
;
Wenjia SUN
;
Ruomei QI
- Publication Type:Journal Article
- Keywords:
ginkgolide B;
human umbilical vein en-dothelial cells;
JAM-A;
Cx43;
vascular permeability;
inflammation
- From:
Chinese Pharmacological Bulletin
2014;(5):646-651
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of ginkgolide B on junctional proteins in ox-LDL-stimulated human umbilical vein endothelial cells ( HUVECs) . Methods After incubation with ginkgolide B ( 0 . 2 ,0 . 4 ,0 . 6 g · L-1 ) for 1 h, HUVECs were treated with ox-LDL (0. 1 g·L-1 ) for 4 h. The expressions of JAM-A and Cx43 were analyzed with Western blot and immunofluo-rescence. The effect of ginkgolide B on vascular per-meability was analyzed by Transwell experiments. Re-sults JAM-A and Cx43 expressions increased by 22%and 24% in ox-LDL-treated HUVECs, respectively. Whereas ginkgolide B significantly decreased JAM-A and Cx43 expressions. LY294002, a specific inhibitor of PI3K, suppressed JAM-A and Cx43 expressions in ox-LDL-stimulated cells. Ginkgolide B potently re-duced monocyte migration in ox-LDL-treated cells. Conclusion Ginkgolide B significantly suppresses JAM-A and Cx43 expressions, and reduces monocyte migration in ox-LDL-stimulated cells. This demon-strates that ginkgolide B can improve vascular permea-bility. The mechanism might be associated with the in-hibition of PI3K/Akt signaling pathway.
