8-bromo-7-methoxychrysin reverses EMT of the sphere-forming cells derived from cervical cancer SiHa cell line
10.3969/j.issn.1001-1978.2014.05.017
- VernacularTitle:8-溴-7-甲氧基白杨素逆转人宫颈癌SiHa细胞系球形成细胞上皮-间叶样表型转化
- Author:
Xiaozheng CAO
;
Xiaohong YANG
;
Jianguo CAO
;
Honglin XIANG
- Publication Type:Journal Article
- Keywords:
cancer stem cells;
cervical cancer;
sphere forming cells;
8-bromo-7-methoxychrysin;
epithelial-mesenchymal transition;
Twist1
- From:
Chinese Pharmacological Bulletin
2014;(5):668-671
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate whether 8-bromo-7-methoxychrysin ( BrMC ) can reverse the epithelial-mesenchymal transition ( EMT) in sphere-forming cells (SFCs) derived from cervical cancer SiHa cell line. Methods Human cervical cancer SiHa cell line was cultured in vitro and then SFCs were obtained from Si-Ha cell line using suspension culture with the stem-condition culture system. After treatment with BrMC for 72 h, the morphology of SFCs of SiHa cell line was observed via monolayer adherence culture. The expres-sion levels of E-cadherin, N-cadherin and Twist1 pro-teins were analyzed using western blot assay. Results SFCs of SiHa cell line presented the mesenchyme-like( spindle-shaped) cell shape and exhibited reduced E-cadherin and elevated N-cadherin protein expres-sion, compared with the parental cells. After treatment with BrMC(1. 0 μmol·L-1 ) for 72 h, SFCs of SiHa cell line changed from spindle-shaped cells in to poly-gon shaped cells under monolayer adherence culture. After treatment with BrMC for 24 h, the expression lev-els of E-cadherin protein were up-regulated and the ex-pression levels of N-cadherin and Twist1 proteins were decreased in SFCs derived from SiHa cell line. Con-clusion BrMC effectively reverses EMT of SFCs de-rived from cervical cancer SiHa cell line, and its mechanism is involved in down-regulating the expres-sion of Twist protein.
