Influence of supernatant from human umbilical cord-derived mesenchymal stem cells on proportions of each human lymphoid subgroup
10.3969/j.issn.1000-484X.2014.05.001
- VernacularTitle:人脐带间充质干细胞培养上清对正常人淋巴细胞各亚群比例的影响
- Author:
Xuan CHEN
;
Yin YUAN
;
Hongwei SHAO
;
Zhiyi LU
;
Liuhua ZHANG
;
Shulin HUANG
- Publication Type:Journal Article
- Keywords:
Human umbilical cord-derived mesenchymal stem cells;
Regulatory T cells;
Immunomodulation
- From:
Chinese Journal of Immunology
2014;(5):577-581
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the impact of human umbilical cord-derived mesenchymal stem cells on the activation ,the survival of human peripheral blood mononuclear cell ( hPBMC) and the proportions of each human lymphoid subgroup .Methods:PB-MC were isolated from healthy donors by density gradient centrifugation , then cultured in MSC-CM as treatment group after being acti-vated by OKT3.Each lymphoid subgroup proportion was analyzed by flow cytometry to observe the difference between treatment and control group .The effect of MSC-CM on activated PBMC for the production of IFN-γand IL-10 were tested by ELISA .The level of ap-optosis was assessed by flow cytometry with Annexin-V/PI as fluorescent marker .Results:Compared with the control group , MSC-CM down-regulated the ratio of CD4 +T cell to CD8 +T cell, and increased the proportion of CD4 +CD25 +CD127low Treg cell, thus other subgroup had no significant difference .MSC-CM inhibited the production of IFN-γby PBMC, but promoted the secretion of IL-10, and protected PBMCs from apoptosis when activated with OKT 3.Conclusion:hUC-MSC may play a role of immunosuppression by promo-ting the proliferation and activation of Treg cell .This kind of inhibitory activity is neither relied direct or indirect contact with the lym -phocytes , nor influenced by inducing immune cells apoptosis .
