Receptor-interacting protein 140 mRNA expression in adipose tissue of a mouse model of high fat diet
10.3969/j.issn.2095-4344.2014.18.018
- VernacularTitle:高脂饮食肥胖模型小鼠脂肪组织受体相互作用蛋白140基因的表达
- Author:
Yanpan ZHAI
;
Luan WANG
;
Ruixin MA
- Publication Type:Journal Article
- Keywords:
insulin;
obesity;
subcutaneous fat;
food habits
- From:
Chinese Journal of Tissue Engineering Research
2014;(18):2892-2896
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Receptor-interacting protein 140-knockout mice are lean and resistant to high fat diet-induced obesity due to an increase in mitochondrial biogenesis, fatty acid oxidation and oxidative phosphorylation.
OBJECTIVE:To study the expression level of receptor-interacting protein 140 mRNA in adipose tissue of high fat diet-induced obese mice and the correlation with insulin resistance.
METHODS:Male C57BL/6J mice were randomly divided into control group and high fat diet group, and fed for 14 weeks separately. The mice of the two groups were weighed. Mice in high fat diet group whose body weight were 20%higher than average weight of control mice were selected as obese mice.
RESULTS AND CONCLUSION:A total of 12 mice in high fat diet group were recruited into the obesity group. The levels of triglycerides, total cholesterol, fast blood glucose and fast insulin in obesity group were significantly higher than those of control group (P<0.05 or P<0.01). The expression of receptor-interacting protein 140 mRNA in adipose was significantly higher in obesity group compared with the controls (P<0.05). Moreover, the expression of receptor-interacting protein 140 mRNA in adipose was positively correlated with triglycerides levels and insulin resistance index (r=0.526, P<0.05;r=0.465, P<0.05), but negatively associated with the levels of total cholesterol, fast blood glucose and fast insulin (P>0.05).
