Regulatory effect of anti-IL-6Rβ(gp130) mAb on IL-6 signaling pathway

VernacularTitle:抗人IL-6 Rβ（gp130）单克隆抗体对IL-6信号分子调控浅析
Author: Xiaowei ZHOU ; Ping MIAO ; Ninan CHENG ; Rong ZHAO ; Liu QIAO ; Qiwen YU ; Jiying ZHANG ; Rong XU ; Dongyi HE ; Lianbo XIAO ; Meisheng LU ; Dongqing ZHANG
Publication Type:Journal Article
Keywords: IL-6; gp130; Anti-IL-6Rβ(gp130) monoclonal antibody; STAT3; RA
From: Chinese Journal of Immunology 2014;(5):639-643
CountryChina
Language:Chinese
Abstract: Objective:To analyse the biological function of anti-IL-6Rβ(gp130) monoclonal antibody and its regulatory effect on IL-6 signaling.Methods:Biological characteristics of anti-IL-6Rβ(gp130) mAb were assessed by Western blot analysis, capture ELISA and peptide ELISA .The phosphorylation of STAT 3 was tested by Western blot analysis in IL-6-stimulated U266/RA-FLS/RA-PBMC with or without anti-IL-6Rβ(gp130) mAb treatment.Results:3 strains of mouse anti-human gp130 mAb were with high affini-ty and different binding epitopes , the kaff of 10A1 was 2.62E-10.In U266, RA-PBMC and RA-SFMC, IL-6 signaling highly activated STAT3 which could be inhibited by anti-gp130 mAb.Conclusion: Anti-IL-6Rβ( gp130 ) mAb might have different binding epitopes and could affect IL-6 stimulated phosphorylation of STAT3, which provides a preliminary experiment for analyse the correlation of IL-6 signaling and RA .