Expression and clinical significance of E3 ubiquitin ligase Iduna in non-small-cell lung cancer
10.3760/cma.j.issn.1673-4904.2014.14.020
- VernacularTitle:E3泛素连接酶Iduna在非小细胞肺癌中的表达及其临床意义
- Author:
Ying GAO
;
Chengyang SONG
;
Linping HUI
;
Ye TIAN
;
Dali TIAN
;
Xueshan QIU
;
Enhua WANG
- Publication Type:Journal Article
- Keywords:
Carcinoma,non-small-cell lung;
Neoplasm metastasis;
Prognosis;
Iduna
- From:
Chinese Journal of Postgraduates of Medicine
2014;37(14):54-58
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mRNA and protein expression of E3 ubiquitin ligase Iduna in non-small-cell lung cancer tissue and para-neoplastic lung tissue,and the correlation of the Iduna expression with clinicopathological factors and prognosis.Methods The expression levels of the Iduna mRNA and protein in non-small-cell lung cancer tissue and para-neoplastic lung tissue were determined by reverse transcriptase-polymerase chain reaction (RT-PCR),Western-Blot and immunohistochemistry respectively,and the correlation of the Iduna expression with clinicopathological factors and prognosis was analyzed.Results RT-PCR and Western-Blot showed the expression levels of the Iduna mRNA and protein in non-small-cell lung cancer tissue (0.468 ± 0.086 and 2.554 ± 0.544) were significantly higher than those in para-neoplastic lung tissue (0.203 ± 0.070 and 1.570 ± 0.316),there were statistical differences (P ＜ 0.05).Immunohistochemistry results showed that Iduna was negative expression in the alveolar epithelium cells,negative or weak positive expression in normal bronchial and positive expression in different degrees in the non-small-cell lung cancer tissue.Iduna high expression rate was negative correlation with tumor differentiation (P =0.002),Iduna high expression rate was positive correlation with large tumor size (P =0.044),TNM staging (P=0.015) and lymph node metastasis (P=0.009).Iduna high expression of I stage non-small-cell lung cancer patients was correlated with poor post-operative survival (P =0.016).Conclusions High expression of Iduna may be related to the process of invasion and metastasis of nonsmall-cell lung cancer.It is possible that Iduna serve as potential markers for predicting prognosis in nonsmall-cell lung cancer.
