Evaluation of long-term-siRNA treatment with HBV transgene mice on inhibit replication of hepatitis B virus
10.3969/j.issn.1000-484X.2014.05.021
- VernacularTitle:对小干扰RNA长期作用HBV转基因小鼠抑制乙肝病毒复制的评价
- Author:
Weiyun ZHANG
;
Zhaohui SUN
;
Guangli REN
;
Yuling SHI
;
Wei LI
;
Rong ZANG
;
Rongzhi TANG
- Publication Type:Journal Article
- Keywords:
siRNA;
HBV transgene mice;
Hepatitis B virus
- From:
Chinese Journal of Immunology
2014;(5):666-668
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigation of the long-term-siRNA treatment with HBV transgene mice on inhibit replication of hepatitis B virus .Methods:The constructed siRNA expressed vectors was transfected HBV transgene mice by hydrodynamics -based in-jection via vena caudalis .Different groups were set including:specificity siRNA groups ( pSilencer5.1/C2,pSilencer4.1/C2,pSilenc-er3.1/C2),PBS group and negative vector group (n=10).The effect was observed in different periods (6 d,21 d,1 months,3 months, 6 months and 9 months after injection ) .HBsAg was analyzed by Chemiluminescence method , HBV-DNA was analyzed by real time quantitative PCR ( RQ-PCR) .Results:Compared with the PBS group , specificity siRNA groups showed decreased levels of HBsAg and HBV-DNA (P<0.05).Negative vector group did not show such changes ,there were no significant differences (P>0.05).Conclu-sion:The siRNA based on the expression vector can suppress the expression and replication of HBV in HBV transgene mice .The inhi-bition effects of long-term-siRNA treatment was specific .
