Effect on expression of macrophages ferroportin and ferritin in mouse alveolar macrophages by mycobacterium tuberculosis
10.3969/j.issn.1000-484X.2014.05.004
- VernacularTitle:不同毒力结核杆菌对感染小鼠肺泡巨噬细胞铁蛋白及铁转运蛋白表达的影响
- Author:
Rui ZHUANG
;
Xia WANG
;
Feng ZHANG
;
Yin BAO
;
Le ZHANG
;
Fang WU
;
Jiangdong WU
;
Chunjun ZHANG
;
Hui ZHANG
;
Wenjuan LI
;
Chen LIANG
;
Chao FAN
;
Wanjiang ZHANG
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
Macrophage;
Ferritin;
Ferroportin
- From:
Chinese Journal of Immunology
2014;(5):591-595
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To discuss the change of ferritin ( Fn) and ferroportin expression quantity and time-related feature in the alveolar macrophages of mice , infected with different virulence of Mycobacterium Tuberculosis infected .Methods:The prepared bacte-ria of H37Rv or BCG were injected intravenously into the mice tails .On the day 1, 3, 5, 7, 9, 11, 13 and 15, the lavage fluids were collected and the alveolar macrophages were obtained from each group of mice .The expression of FPN and Fn were detected with ELISA and /or Western blot analysis .Results:The expression of Fn in the group of either H 37Rv or BCG infected mice was decreased on the day 7, 9 and 11, and was lowest on the day 7, which showed significantly statistical difference compared to that on the other days (P<0.05).The expression of FNP in the infected mouse macrophage was decreased gradually , which was obvious on the day 5. The expression levels reached to the lowest on the day 7 and 9.The expression was much lower than that in the negative control group (P<0.05).Conclusion:The expression of Fn and FPN in macrophages isolated from lungs of mice infected with Mycobacterium tu -berculosis H37Rv or BCG become decreased , and there is no difference between these two infected mouse groups .
