Comparison of the rat models of liver cancer spleen deficiency established by Dachengqi and Xiaochengqi decoctions
10.3969/j.issn.1005-4847.2014.02.005
- VernacularTitle:大小承气汤制作大鼠脾虚肝癌模型的比较
- Author:
Baoguo SUN
;
Ting XIANG
;
Yulong LI
;
Zexiong CHEN
- Publication Type:Journal Article
- Keywords:
Spleen deficiency;
Liver cancer;
Dachengqi decoction;
Xiaochengqi decoction;
Animal model;
Rat
- From:
Acta Laboratorium Animalis Scientia Sinica
2014;(2):20-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the establishing methods and differences of rat models of spleen deficiency and spleen deficiency liver cancer using the traditional Chinese medicine Dachengqi and Xiaochengqi decoctions .Methods Spleen-deficiency rat models were developed by multifactor methods:bitter-cold purgation ( Dachengqi or Xiaochengqi de-coction), cold-wet environment, tiredness, and fasting on alternate days for 30 days.Seven days after spleen-deficiency modeled,liver cancer in the spleen-deficiency rats and normal rats was developed by subcutaneously inoculation of Walker -256 carcinoma cell line in nude mice and then transplanted into rat livers .Liver cancer models were observed for 35 days. Sixty 3-week old male Wistar rats were randomly distributed into 4 groups: normal group , liver cancer model group , and Dachengqi and Xiaochengqi decoction groups .Degree of spleen deficiency , changes of the body-weight, survival time and tumor formation were recorded .Results Spleen deficiency rat models were successfully established .The weight gain of rats in the spleen-deficiency groups was significantly inhibited (P<0.01), and during the first 20 days (but not later) the average body weight of the Dachengqi decoction group was significantly higher than that of the Xiaochengqi decoction group (P<0.05).Spleen-deficiency scores of rats in the Xiaochengqi and Dachengqi groups were higher than those in the blank tumor group, especially in the Xiaochengqi group (P<0.01).The total tumor formation rate was 91.1%and 80%in the blank tumor groups , and 93.3%in both Xiaochengqi and Dachengqi groups , respectively .The average survival time of Xi-aochengqi group was lower than that of the blank tumor and Dachengqi groups ( P<0.01 and P<0.05 ) .The cumulative survival rate of the Xiaochengqi group and rats with a higher spleen-deficiency score was lower than that of the other groups (P<0.05).Conclusions Xiaochengqi decoction may induce spleen deficiency more seriously than Dachengqi decoction , and spleen deficiency may be an important unfavorable prognostic factor for rat models of liver cancer .
