Screening and research of homologous peptide with adenovirus receptor
10.3969/j.issn.1000-484X.2014.05.018
- VernacularTitle:7型腺病毒受体同源性多肽的筛选和研究
- Author:
Yuansheng XU
;
Xingui TIAN
;
Minglong LIU
;
Chenyang LI
;
Rong ZHOU
- Publication Type:Journal Article
- Keywords:
Adenovirus type 7;
Receptor;
Peptide;
Immunofluorescence
- From:
Chinese Journal of Immunology
2014;(5):651-653
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To screen the binding peptide against adenovirus type 7(Ad7) and evaluate the relevance with the ade-novirus receptor .Methods:Binding peptide against Ad 7 was screened by panning the phage display 12 peptides library .The antibody against the selected peptide was prepared and was used to study the binding to the membrane by immunofluorescence technique .Re-sults:Using Ad7 as the target protein , GTS09 peptide was selected from the phage display 12 peptides library by biopanning .GTS09-phage complex could significantly bind Ad 7, with the affinity constant up to 1.93 ×1010 L/mol;at the same time, immunofluorescence showed that antibody of GTS09 could specifically bind to membrane of 293 cell.Conclusion: Antibody against GTS09 peptide could specifically bind to membrane of 293 cell,which shows that the peptide may presumably have homology with the cell receptors of Ad 7.
