Expression pattern of transcription factor Olig2 in cuprizone-induced mouse model of acute demyelination
10.3969/j.issn.1005-4847.2014.02.008
- VernacularTitle:Olig2在 cuprizone 介导的脱髓鞘动物模型中的表达变化
- Author:
Liping CHEN
;
Jing ZHANG
;
Shunli MA
;
Zhenfei LI
;
Jinli ZHANG
;
Mei DONG
;
Mingyue SHAN
;
Li GUO
- Publication Type:Journal Article
- Keywords:
Multiple sclerosis;
Cuprizone;
Remyelination;
Olig2;
Mice
- From:
Acta Laboratorium Animalis Scientia Sinica
2014;(2):36-39
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression pattern of transcription factor Olig 2 in cuprizone-induced mouse model of acute demyelination .Methods C57BL/6 mice were fed with 0.2%cuprizone to induce acute demyelina-tion.Immunofluorescence and qRT-PCR were used, and Olig2, MBP and GFAP were detected in the brain tissues of con-trol group and cuprizone-treated groups for 6 weeks and recovery for 2 weeks.Results Severe demyelination occurred in the corpus callosum following 6-weeks exposure to cuprizone , while remyelination was detected in the white matter after the mice were given diet without cuprizone .In the normal mice , Olig2 was expressed in a low level , while the experessions of Olig2 and GFAP were significantly increased , and Olig2 +/GFAP+cells were detected after demyelination .But the expres-sion of MBP was below the normal level with demyelination .After recovery for 2 weeks, the experession of Olig2 was lower, but the experessions of MBP and GFAP were increased .Conclusions Olig2 may play an important role in the glial differ-entiation from neural progenitor cells into active astrocytes , and in the glial scar formation .
