Regulation of bone marrow mesenchymal stem cells on lymphocyte proliferation of type Ⅰdiabetic rats
10.3969/j.issn.1000-484X.2014.05.024
- VernacularTitle:骨髓间充质干细胞对Ⅰ型糖尿病大鼠淋巴细胞增殖的调节作用
- Author:
Guixing ZHENG
;
Yiquan LI
;
Xiaoping WEI
;
Jie WU
;
Jun HUANG
- Publication Type:Journal Article
- Keywords:
Bone marrow mesenchymal stem cells;
Type Ⅰdiabetic;
Rat;
Lymphocytes
- From:
Chinese Journal of Immunology
2014;(5):677-680
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the regulation mechanisms of the bone marrow mesenchymal stem cells on lymphocyte pro -liferation of type I diabetic rats .Methods:The rat bone marrow mesenchymal stem cells were isolated , cultured and identified and the effect on lymphocyte proliferation of type Ⅰdiabetic rat was observed by MTT assay , and analyze the CD 4 +CD25 +regulatory T cell ra-tio, cell cycle and apoptosis of type I diabetes rat by flow cytometric .Results:B and C groups was significantly lower than the absor-bance values of group A,the differences between the data were statistically significant (P<0.05), C group was significantly lower than group B absorbance values, the difference was significant (P<0.05);the CD4 +CD25 +regulatory T cells of B and C groups were sig-nificantly higher than group A, the differences of the data were statistically significant (P<0.05), the CD4 +CD25 +regulatory T cell ratio of C group significantly higher than that group B , the differences were statistically significant (P<0.05);the apoptosis levels of B and C groups were significantly higher than group A , the differences were statistically significant (P<0.05), the apoptosis levels of C group were significantly higher in group B , the differences were statistically significant (P<0.05).Conclusion:Bone marrow mesen-chymal stem cells can significantly inhibit lymphocyte proliferation of type Ⅰdiabetic rats, and it may regulate CD4 +CD25 +regulatory T cells, promote apoptosis, thereby affecting the immune function of T lymphocytes , and play its rejection.
