Establishment of a rat model of lumbar nerve root compression via modified unilateral hemilaminectomy approach
10.3969/j.issn.1005-4847.2014.02.011
- VernacularTitle:改良半椎板切除法建立大鼠腰神经根压迫模型
- Author:
Jingjing TANG
;
De LIANG
;
Xiaobing JIANG
;
Jingyong DING
;
Zhidong YANG
;
Zhensong YAO
;
Shuncong ZHANG
- Publication Type:Journal Article
- Keywords:
Unilateral hemilaminectomy;
Never root compression;
Model;
Rat
- From:
Acta Laboratorium Animalis Scientia Sinica
2014;(2):48-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the characteristics and advantages of the rat model of lumbar nerve root com -pression established via modified hemilaminectomy approach .Methods Forty Sprague Dawley ( SD) rats were randomly divided into two groups ( experimental group and control group ) for establishing a rat model of lumbar nerve root compres-sion.Modified hemilaminectomy apprpoach was used in the experimental group , while total laminectomy was applied in the control group.Duration of operation, estimated blood loss, wound healing status, postoperative mortality, motor function of the lower extremities , pathological changes and the gray level of cytoplasmic TNF-αand IL-1 expression were observed to e-valuate the characteristics and reliability of the two operation approaches in establishing a rat model of lumbar nerve root compression.Results Compared with the control group , duration of operation , estimated blood loss , wound healing sta-tus, postoperative mortality were significantly better in the experimental group (P<0.01), whereas there was non-signifi-cant difference between two groups with regard to motor function of the lower extremities , pathological changes and gray lev-el of the cytoplasmic expression of TNF-αand IL-1 between the two groups .Moreover, the soft tissue structural alterations were reduced in the experimental group , which might be helpful for the rat postoperative rehabilitation .Conclusions Modified hemilaminectomy approach is a reliable alternative method for establishing a rat model of lumbar nerve root com -pression, which is beneficial to shorten the operative time , improve wound healing status , reduce estimated blood loss and damages of soft tissue , and decrease mortality rate .Moreover , this modified method is more consistent with the principles of animal ethics .
