Comparison of the effect of three cryoprotectants on vitrification-cryopreservation of mouse epididymis
10.3969/j.issn.1005-4847.2014.02.014
- VernacularTitle:三种不同冷冻保护剂对小鼠附睾冷冻效果的比较
- Author:
Miao LI
;
Lili YU
;
Yibao ZHANG
;
Sujing QIANG
;
Lijun LIU
;
Ping XU
- Publication Type:Journal Article
- Keywords:
Mouse;
Epididymis;
Cryoprotectant solution;
Freezing efficiency;
Vitrification
- From:
Acta Laboratorium Animalis Scientia Sinica
2014;(2):62-65
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effect of three cryoprotectants on vitrification-cryopreservation of C57BL/6J mouse epididymis.Method Epididymises from 6-8-week old male C57BL/6J mice (age) were cryopreserved using DMSO, PROH and R18S3 cryoprotectant solution and thawed , respectively.The morphology of sperm from thawed epididy-mis, the rate of post-thawing survival and reproductive capacity were determined to evaluate the freezing efficiency of the three cryoprotectants .Results The sperms from thawed epididymis of the three groups were all well-preserved structural-ly.The survival rate of sperms was 88.17%, 61.17% and 16.83% in the PROH, DMSO and R18S3 cryoprotectant solu-tions, respectively, and there were significant differences between the three cryopreservation groups (P<0.05 for all). The number of pups from the DMSO , PROH and R18S3 groups were 13, 8 and 17 mice after ICSI and embryo implantation , respectively.Conclusions All the three cryoprotectants DMSO , PROH and R18S3 solutions are suitable for vitrification-cryopreservation of C57BL/6J mouse epididymis.But PROH is the preference of these cryoprotectant solutions .
