Detection and isolation of murine norovirus in laboratory mice in Shanghai area
10.3969/j.issn.1005-4847.2014.02.018
- VernacularTitle:上海地区小鼠诺瓦克病毒的检测分析及病毒分离
- Author:
Qin LIU
;
Xiaofeng WEI
;
Lili TIAN
;
Cheng GAO
- Publication Type:Journal Article
- Keywords:
Murine norovirus (MNV);
Natural infection;
RT-PCR;
ELISA
- From:
Acta Laboratorium Animalis Scientia Sinica
2014;(2):80-85
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the natural infection status of murine norovirus ( MNV) in laboratory mice in Shanghai area and isolate MNV from mouse cecal feces .Methods To collect cecal contents and serum samples from 319 specific pathogen-free ( SPF) mice coming from different research institutions .Reverse transcription-polymerase chain reaction ( RT-PCR) and enzyme linked immunosorbent assay ( ELISA) were used to detect MNV infection in the mice , re-spectively.The positive stool samples were diluted and filtered through 0.22 μm membrane, inoculated into RAW 264.7 cells, and then identified by RT-PCR.Results There were 95 positive results in the 319 cecal samples by RT-PCR, and the positive rate was 29.78%.Among 180 serum samples which were tested by RT-PCR, 70 samples were positive by ELISA, and the positive rate was 38.89%.The infected RAW 264.7 cells showed cytopathic effect ( CPE) within 72 h. After 3 times of freezing and thawing , RT-PCR obtained a 187 bp band.Conclusions The results from the present study show that there is a high natural infection rate of MNV in laboratory mice in Shanghai area , and the strict breeding manage-ment must be strengthened .
