Detection of mecA gene in Staphylococcus aureus and its correlation with drug-resistance
10.3969/j.issn.1671-8348.2014.11.011
- VernacularTitle:金黄色葡萄球菌中mec A基因的检测及其耐药相关性
- Author:
Xiaofeng CHEN
;
Yang PENG
;
Jiaqi BI
;
Zhengjiang YAO
- Publication Type:Journal Article
- Keywords:
staphylococcus aureue;
mecA gene;
drug resistance
- From:
Chongqing Medicine
2014;(11):1312-1314
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mecA gene expression lvevl in clinically isolated Staphylococcus aureus (SA) strains and its correlation with drug resistance .Methods Clinically isolated 186 SA strains were collected .The K-P method was adopted to conduct the durg sensitivity test .Then DNA of these strains was extracted and the mecA gene was amplified by using PCR .Results The detection rate of methicillin-resistant SA(MRSA) was 30 .65% (57/186) ,the positive mecA gene was detected in 56 strains of MRSA and 10 strains of methicillin susceptible S .aureus(MSSA) among 129 strains of MSSA ;except susceptible to vancomycin and linezolid ,the resistance rate of MRSA to ther antibacterial durgs were higher than that of MSSA ,the resistance rate to antibac-terial durgs in the strains carrying mecA gene was higher than that in the strains without carrying mecA gene ,ther difference be-tween them had statistical significance (P<0 .05) .Conclusion Clinically isolated SA strains carrying mecA gene are resistant to multiple antibacterial drugs ,which indicating that mecA gene play an important role in SA drug-resistance mechanism .
