A morphological and cytotoxic study on nucleus pulposus ECM-derived scaffold fabricated from rabbit
10.3969/j.issn.1006-5725.2014.07.011
- VernacularTitle:脱细胞兔髓核支架的形态学及体外细胞毒性研究
- Author:
Wei CAO
;
Hai LV
;
Chusong ZHOU
- Publication Type:Journal Article
- Keywords:
Scaffold;
Nucleus pulposus;
Decellularization;
Morphology;
Cytotoxicity
- From:
The Journal of Practical Medicine
2014;(7):1041-1044
- CountryChina
- Language:Chinese
-
Abstract:
Objective To use detergents and nucleic acid enzyme to prepare scaffold of extracellular ma-trix , then assess the morphological and cytotoxic changes in vitro , and explore the feasibility of this type of scaffold as an ideal tissue-engineering scaffold. Methods Fifty pieces of fresh nucleus pulposus were randomly divided into a fresh control group and a decellularized group. The specimens in decellularized group were treated with 0.3%Tri-ton X-100, 0.5%sodium deoxycholate, and nuclease for 24 h. Morphological changes were studied by macroscopy, pathological staining and scanning electron microscopy. Cytotoxicity was determined by CCK-8 and LIVE/DEAD Viability/Cytotoxicity Assay Kit in vitro. Results The shape of scaffold was maintained,and the extracellular ma-trix was presented while the cells disappeared after decellularization. As compared with the fresh tissue , the scaffold and its extracts had no cytotoxicity to rabbit bone marrow stem cells. Conclusions Almost all the cells have been removed while the extracellular matrix is reserved , and the scaffold has no cytotoxicity to the seed cells. The decel-lularized scaffold can be used as an ideal substance to fabricate tissue-engineering nucleus pulposus.
