Mechanism of inhibition effect of HGF on the expression of CTGF in myotubes induced by TGF-β1
10.3969/j.issn.1006-5725.2014.07.012
- VernacularTitle:肝细胞生长因子抑制转化生长因子-β1诱导肌管的结缔组织生长因子表达作用机制研究
- Author:
Yue SUN
;
Siwen XIA
;
Shicai CHEN
;
Donghui CHEN
;
Hongliang ZHENG
- Publication Type:Journal Article
- Keywords:
Hepatocyte growth factor;
Transforming growth factor-β1;
Myotubes;
Connective tissue growth factor
- From:
The Journal of Practical Medicine
2014;(7):1045-1047
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence and mechanism of hepatocyte growth factor (HGF) on myotube phenotype by myotube transdifferentiation induced by transforming growth factor-β1 (TGF-β1). Methods C2C12 cells were cultured in differentiation medium to induce myotubes formation. The cells were randomly devided into 3 groups. The control group without growth factor interruption. The induction group was supplemented with TGF-β1 (5 ng/mL) while the inhibition group was supplenmented with both TGF-β1 (5 ng/mL) and HGF (30 ng/mL). After 12 hours, the expressions of connective tissue growth factor (CTGF) protein in myotubes were detected by Western blot, the levels of CTGF mRNA were measured by RT-PCR. Results Compared to the control group, the protein and mRNA levels of CTGF significantly increased in TGF-β1 treated group , whereas the protein and mRNA levels of CTGF were significantly lower in inhibition group than those in induction group (P < 0.05). Conclusion HGF can inhibit the effect of TGF-β1 on the expression of CTGF in myotubes , which provides the evidences on the study of skeletal muscle cell transdifferentiation.
