Overexpression of the tumor suppressor gene PTEN inhibits the phosphorylation of Akt in activated hepatic stellate cells in vitro
10.3969/j.issn.1006-5725.2014.07.019
- VernacularTitle:PTEN过表达抑制体外活化肝星状细胞Akt的磷酸化
- Author:
Lisen HAO
;
Xiaolan ZHANG
;
Changzhen REN
;
Liwen LI
;
Jing WANG
;
Yanbo MO
;
Rongrong BIAN
;
Yue WEI
;
Jiaqi ZHANG
;
Yuling LIU
- Publication Type:Journal Article
- Keywords:
Hepatic stellate cell;
PTEN;
Akt;
Signaling transduction
- From:
The Journal of Practical Medicine
2014;(7):1069-1072
- CountryChina
- Language:Chinese
-
Abstract:
Objective Using an adenoviral vector , the wild-type PTEN gene was transduced into activated hepatic stellate cell (HSC) in vitro and the phosphorylation status of Akt were investigated. Methods The wild type PTEN gene was transduced into activated HSC in vitro mediated by adenoviral vector. The expressions of PTEN and total Akt in HSC were measured by Western blot and Real-time fluorescent quantitation PCR. And the expressions of phosphorylated Akt (Thr308) in HSC was determined by Western blot. Results The data showed that exogenous wild type PTEN gene was successfully transduced and expressed in activated HSC in vitro. The over-expression of wild type PTEN resulted in the significant down-regulated expression of phosphorylated Akt (Thr308) in activated HSC (P < 0.01). But no significant defferences were found in the expression of total Akt in activated HSC at both transcriptional and translational levels(P>0.50). Conclusions The overexpression of wild-type PTEN can negatively regulate PI3K/Akt signaling transduction by inhibiting the phosphorylation of Akt in activated HSC in vitro.
