Comparative evaluation of cytomegalovirus DNA stability in EDTA plasma over a 21 day period
10.3969/j.issn.1006-5725.2014.07.045
- VernacularTitle:存储时间对血浆中巨细胞病毒DNA稳定性的影响
- Author:
Li XIE
;
Zhen YI
;
Jian WANG
;
Zhijian CHEN
;
Juanjuan XU
;
Shan LI
;
Xue QIN
- Publication Type:Journal Article
- Keywords:
Prolong period;
Cytomegalovirus;
DNA;
Stability
- From:
The Journal of Practical Medicine
2014;(7):1151-1153
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze CMV DNA stability of 30 EDTA plasma samples in the order of magnitude between 300 and 100 000 copies/mL over a 21 day period. Methods Thirty plasma samples were grouped into three categories according to the CMV DNA loads , including low CMV DNA contents , intermediate CMV DNA loads and high CMV DNA loads. Ten milliliters of whole blood was freshly collected from each patient. Plasma samples without hemolysis were divided into 1-ml aliquots. One aliquot was processed immediately (Day 0) for baseline PCR assays. The remaining aliquots were then processed after one , two, three, seven, 14 or 21 day of storage at 4℃. Results There was no significant difference between the mean of the difference time point in viral loads following storage at 4 ℃ by paired-samples t test, including Day 1 compared to Day 0 (t = 1.654, P =0.109), Day 2 compared to Day 0 (t = 1.487, P = 0.148), Day 3 compared to Day 0 (t = 1.609, P = 0.118), Day 7 compared to Day 0 (t=0.831, P=0.413), Day 14 compared to Day 0 (t=1.721, P=0.096), and Day 21 compared to Day 0 (t=0.244, P=0.810). Conclusion The concentration of CMV DNA in all samples stored at 4 ℃ for 21 days did not differ significantly from the baseline viral load ,and it was not observed the trend in continued degradation in different time point (Day 1, 2, 3, 7 and 14).
