Comparison and evaluation of three methods to establish mouse models of intestinal infection with multidrug-resistant Pseudomonas aeruginosa
10.3969/j.issn.1005-4847.2014.02.016
- VernacularTitle:不同方法建立多重耐药铜绿假单胞菌肠道感染动物模型的比较和评价
- Author:
Huazhong HAN
;
Jun YANG
- Publication Type:Journal Article
- Keywords:
Multi-drug resistance;
Pseudomonas aeruginosa;
Intestinal infection;
Intestinal barrier;
Mouse
- From:
Acta Laboratorium Animalis Scientia Sinica
2014;(2):70-74
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the establishment methods of animal models of intestinal multidrug-resistant bacterial (Pseudomonas aeruginosa, PA) infection, and to provide a good model for experimental research and treatment for infection caused by drug-resistant bacteria .Methods Male BALB/c mice were divided into four groups:Normal con-trol group, MDR-PA group, MDR-PA +antibiotics group, and MDR-PA +fasting group.They were given saline, PA suspension, PA suspension after free drinking with ceftriaxone water and PA suspension after fasting , respectively , by ga-vage.Colon tissues were collected for pathological examination and inflammation scoring .The expressions of TNF-αand INF-γin the colon tissue was assessed at the end of the experiment .Results Pathology showed different degrees of inflam-matory changes in the colon tissues .Compared with the normal control group , the expressions of TNF-αand INF-γhad sig-nificant differences with that of experimental groups .Conclusions All the three methods used in this experiment can be successfully used to establish mouse models with intestinal infection caused by multidrug-resistant Pseudomonas aeruginosa, and may be chosen for experimental studies according to different research purposes .
