Research on gout-related SNP genotyping by improved tri-primer allele gene amplification
10.3969/j.issn.1671-8348.2014.12.023
- VernacularTitle:采用改进的三引物等位基因扩增法对痛风相关SN P位点的分型研究
- Author:
Chenna ZHENG
;
Qingyao WANG
;
Yuxiang HUANG
;
Guihua YE
;
Chaochen XU
;
Huiyong YANG
- Publication Type:Journal Article
- Keywords:
tri-primer amplification;
alleles;
gene amplification;
gout;
polymorphism,single nucleotide
- From:
Chongqing Medicine
2014;(12):1476-1479
- CountryChina
- Language:Chinese
-
Abstract:
Objective To improve the tri-primer allele gene amplification for realizing the single nucleotide polymorphisms (SNP) genotyping of the peripheral blood sample .Methods Aiming at the peripheral blood samples with the clinical usual antico-agulation processing by EDTA ,heparin and citrate ,with the locus rs1165205 as the target site ,the buffer solution(YW) suitable for whole blood was prepared and the PCR amplification system and the amplification condition were optimized for realizing the detec-tion of SNP genotyping .Results The genotyping results of locus rs1165205 by improved tri-primer allele gene amplification method were consistent with the results of the Sanger sequencing method ,and the peripheral blood samples treated by different anticoagu-lant were genotyped by the improved tri-primer ASA .Among 80 samples ,various genotypes of locus rs1165205 had no statistical differences in the distribution between the gout population and non-gout population(P= 0 .335) .Conclusion The improved tri-primer allele gene amplification method can be adopted to conduct the rapid genotyping research on gout SNP locus of the peripheral blood samples with the clinical usual anticoagulation processing .
