Preliminary recognition of stem cells surface markers in hepatoblastoma
10.3969/j.issn.1671-8348.2014.10.020
- VernacularTitle:肝母细胞瘤中的干细胞表面标记物的初步识别
- Author:
Shouhua ZHANG
;
Juhua XIAO
;
Qingqiang DENG
;
Hongyan XU
;
Ming LUO
;
Weilong CHEN
;
Jun LEI
;
Qiang TAO
- Publication Type:Journal Article
- Keywords:
neoplastic stem cells;
hepatoblastoma;
antigens,CD34;
antigens,Thy-1;
proto-oncogene proteins c-kit
- From:
Chongqing Medicine
2014;(10):1208-1210
- CountryChina
- Language:Chinese
-
Abstract:
Objective To preliminarily screen the neoplastic stem cell (NSC) related surface markers combination and to under-stand the NSC distribution in hepatoblastoma(HB) .Methods The children cases of HB undergone the surgical therapy in the Jian-gxi Provincial Children′s Hospital were selected .The immunohistochemical method was adopted for observing the expression and distribution of NSC-related markers CD34 ,Thy-1 ,c-kit ,CD56 and stem cell factor(SCF) in the HB tissue and the normal hepatic tissue away from the edge of tumor tissue 3cm outside .Results Thy-1 and c-kit were sporadically distributed in the HB tissue and mainly focued on the portal area ,but did not exressed in the normal liver tissue ;the expression of CD34 and SCF in HB was signifi-cantly higher than that in the normal liver tissue(P<0 .05) ,in which CD34 was mainly distributed in the endothelial system and SCF was mainly expressed in the portal area ;CD56 was expressed in the tissue of the clustered nerve fibers ,the expression differ-ence between them had no statistical significance (P>0 .05) .Conclusion The different NSC related surface markers are distributed in the HB tissue and focus on the specific areas .The positive cells of Thy-1/c-kit expression may play a role in the HB occurrence .
