Protective effect of combination of cryptotanshinone and donepezil on amyloid-βprotein induced apoptosis in SH-SY5Ycells

VernacularTitle:隐丹参酮与多奈哌齐合用对β-淀粉样蛋白诱导S H-SY5Y 细胞凋亡的保护作用
Author: Zhengrong MEI ; Fangyan ZHANG ; Zhonghong WU ; Zhonglin ZHANG
Publication Type:Journal Article
Keywords: Alzheimer disease; cryptotanshinone; donepezil; apoptosis
From: Chongqing Medicine 2014;(10):1211-1213
CountryChina
Language:Chinese
Abstract: Objective To investigate the protective effect of cryptotanshinone (CTS) and donepezil(DON) on amyloid-βprotein (Aβ)-induced apoptosis in SH-SY5Y cells .Methods SH-SY5Y cells were cultured in vitro for establishing the Alzheimer disease (AD) model .The cell viability was detected by the MTT assay .The apoptosis rate was measured by Hoechst 33342 and the expres-sion of Bcl-2 and Bax was detected by Western blot .Results CTS ,DON and their combination could obviously alleviate Aβ-caused injury of SH-SY5Y cells ,increase the cell survival rate ,remarkably up-regulate the expression of Bcl-2 protein ,decrease the expres-sion of Bax protein and inhibit the apoptosis .The effect of the CTS and DON combination for inhibiting apoptosis was significantly stronger than that of the single use of CTS and DON ,the difference had statistical significance(P<0 .05) .Conclusion The combi-nation of CTS and DON has the synergistic protective effect on Aβ-caused injury in SH-SY5Y cells ,its mechanisms may be related with the cooperation regulation of the expression of apoptosis related gene Bcl-2 protein family .