Research on cellular receptors associated with HBV preS 1 adhesion
10.3969/j.issn.1671-8348.2014.10.024
- VernacularTitle:与 HBV preS1黏附相关的细胞受体的研究
- Author:
Lianghong WANG
;
Li YANG
;
Wei PAN
;
Xing LI
;
Guozhen YANG
- Publication Type:Journal Article
- Keywords:
hepatitis B virus;
immunomagnetic beads;
candidate receptor of preS1 of hepatitis B virus;
glucose regulated protein precursor 78
- From:
Chongqing Medicine
2014;(10):1221-1223
- CountryChina
- Language:Chinese
-
Abstract:
Objective To seek the specific receptors associated with hepatitis B virus (HBV) adhesion by separating the binding protein of the HBV preS1 region in HepG2 and performing the mass spectrometry .Methods The immunomagnetic bead method was adopted to separate HepG2 membrane protein combined with preS1 peptide fragment and the binding protein was separated by the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ,then the destination strips was analyzed by LC-MS/MS mass spectrometry and retrieved by the database .Results 16 bands were separated from HepG2 membrane proteins combined with preS1 by SDS-PAGE ;14 kinds of proteins were identified from 6 bands with better repeatability separated from HepG 2 membrane proteins combined with preS1 .Conclusion Protein analyzed by the mass spectrometry is mainly related with the material transport , cellular signal transduction ,antigen presentation ,immune regulation and energy metabolism .
