Screening serum biomarkers in gastric cancer by iTRAQ-Coupled 2D-LC-MS/MS
10.3969/j.issn.1006-5725.2014.07.046
- VernacularTitle:iTRAQ结合质谱筛选胃癌血清生物标记物
- Author:
Chunhui WEI
;
Mingyu LAI
;
Xijing MO
- Publication Type:Journal Article
- Keywords:
Gastric cancer;
iTRAQ;
Serum biomarkers
- From:
The Journal of Practical Medicine
2014;(7):1154-1157
- CountryChina
- Language:Chinese
-
Abstract:
Objective To apply the Isobaric tags for relative and absolute quantitation (iTRAQ) to identify proteins differentially in serums of gastric cancer (GC) patients, gastric mucosal intestinal metaplasia (IM) patients and normal control population. Methods The study included serum samples from GC group, IM group and normal control group with 45 cases each group,Proteins differentially regulated in serums were identified by the iTRAQ coupled with two-dimensional liquid chromatography/tandem mass spectrometry (2D-LC-MS/MS) technology. Results 10 540 unique peptides , of which correspond to a set of 199 proteins were identified , 23 proteins were over expressed and 14 proteins were underexpressed in GC and IM.Nine immune response proteins such as Lipopolysaccharide-binding protein,C-reactive protein and Ficolin-3 were found in the differential proteins, implying a close linkage between immune response and GC. Conclusions iTRAQ technology may help to identify novel serum markers for early diagnosis of GC.
