Effects of SAHA combined with PTX on survival and apoptosis of human paclitaxel-resistant ovarian cancer cells
10.7644/j.issn.1674-9960.2014.03.010
- VernacularTitle:辛二酰苯胺异羟肟酸联合紫杉醇对卵巢癌紫杉醇耐药细胞存活与凋亡的影响
- Author:
Zhaohui LIU
;
Yuanlin LIU
;
Junfeng LI
;
Yue ZHAO
;
Ying TONG
;
Yi ZHANG
- Publication Type:Journal Article
- Keywords:
SAHA;
survival;
apoptosis;
synergism;
paclitaxel-resistant human ovarian cancer cell OC 3/P
- From:
Military Medical Sciences
2014;(3):203-206
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of SAHA or/and PTX on survival and apoptosis of human paclitaxel-resist-ant ovarian cancer OC3/P cells, and explore whether the combination of two drugs has a synergistic effect .Methods The morphology of OC3/P cells in different drug-groups was observed by inverted microscope .Cell viability was evaluated by MTT assay.The apoptosis rate was analyzed by Annexin V-FITC/PI assay.Results The morphology change of OC 3/P cells treated with different drug was observed by inverted microscope , and the change in combination group was more signif-icant than one drug alone group .The result of cell survival measured by MTT assay showed that inhibition rate of combina -tion group was more higher than one drug alone group (P<0.05).The analysis of factorial design and gold formula method all proved that the two drugs had synergy .Further the result of flow cytometry showed that apoptosis rate in combination group was significantly higher than SAHA or PTX alone group (P<0.05).Conclusion SAHA and PTX can inhibit the survival and induce apoptosis of OC 3/P cells, and two drugs have synergistic antitumor effects .
