Study the effect of Pingchuan formula on Rho/Rock signaling pathway in the mice of asthmatic model
10.3760/cma.j.issn.1673-4246.2014.05.013
- VernacularTitle:平喘方对哮喘模型小鼠Rho/Rock信号通路表达的影响
- Author:
Jie WU
;
Jianer YU
;
Zheng XUE
;
Xinguang ZHANG
;
Li BAI
- Publication Type:Journal Article
- Keywords:
Asthma;
Pingchuan Formula;
The Rho/Rock signaling pathway
- From:
International Journal of Traditional Chinese Medicine
2014;(5):435-437
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Pingchuan Formula(PCF)on Rho/Rock signaling pathway in the mice of asthmatic model, explore the mechanism of PCF on asthma. Methods Forty BALB/C mice were randomly divided into four groups:a control group, a model group,a dexamethasone group and a PCF group. After treated with medicine for 4 weeks, albumen expression of RhoA and RockI in lung tissues were detected by using Western blot, mRNA expressions of RhoA and RockI were detected by using Real-time PCR. Results The albumen and the mRNA expression of RhoA and RockI in the model group were(1.05± 0.20),(1.06±0.08),(6.60±1.09), (6.53±1.84), the control group were(0.76±0.08), (0.84±0.14), (3.82± 1.77), (3.65±1.46),there was a significant difference between two groups(P<0.01). The albumen and the mRNA expression of RhoA and RockI in the dexamethasone group were(0.78±0.11), (0.87±0.32), (4.19± 2.33), (4.09±1.08), there was a significant difference compared with the model group(P<0.01). The PCF group were(0.86±0.12), (0.93±0.14), (4.38±2.01), (4.50±1.13), there was a difference compared with the model group(P<0.05). These was no difference between the dexamethasone group and the PCF group(P>0.05). Conclusion The expressions of Rho/Rock signaling pathway were controlled significantly by PCF, and the curative effect was similar to dexamethasone.
