Relationship between miR-486-5p and biological behavior of gastric carcinoma SGC7901 cells
10.3969/j.issn.1007-3969.2014.04.006
- VernacularTitle:miR-486-5p对胃癌细胞株SGC7901生物学行为的影响
- Author:
Ming LI
;
Haifeng LIAN
;
Chengxia LIU
;
Yingbin HU
;
Youjie LI
- Publication Type:Journal Article
- Keywords:
Gastric carcinoma;
miR-486-5p;
Proliferation;
Apoptosis;
Migration abilities
- From:China Oncology
2014;(4):273-278
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:MicroRNA-486-5p (miR-486-5p) has been demonstrated to play an important role in many kinds of tumor, however, there are few reports about the relationship between miRNA-486-5p in gastric carcinoma. This study was aimed to explore the effect of miR-486-5p on the proliferation, apoptosis and migration abilities of the human gastric cancer cell line SGC7901.Methods:Quantitative real-time PCR (qRT-PCR) analysis was performed to detect the expression of miR-486-5p in the SGC7901 and GES-1 cells, miR-486-5p over-expressing plasmid was constructed and transfected into the human gastric carcinoma cell line SGC7901 using LipofectamineTM2000. The expression of miR-486-5p of the transfected cells was measured by qRT-PCR, the proliferation level of SGC7901 cells was detected by MTT method, the apoptosis rate of the cells was measured by lfow cytometry and the in vitro migration abilities of SGC7901 cells by transwell test. Results:The miR-486-5p expression in SGC7901 cells was down-regulated compared with GES-1 cells. The expression of miR-486-5p in SGC7901 cells that was transfected miR-486-5p over-expressing plasmid was obviously up-regulated. The proliferation and migration abilities of SGC7901 cells were inhibited signiifcantly, and the apoptosis rate of the cells increased. Conclusion:miR-486-5p can effectively suppress the proliferation and in vitro migration abilities of SGC7901 cells, indicating that miR-486-5p might be used as a target for molecular therapy of gastric cancer.
